g^`;B" }3}{} w0Y ?E6*Ef Forestry Studies in China 2009 11 (3): 179-184 ISSN: 1008-1321 CN: 11-3919/S
Vm3e6Y,K BGe&c,feIc Information & service
|afzW=8' This article
S4'\=w# Supporting info
C>A*L4c]F PDF(0KB)
W5&;PkhQ6 [ Full HTML](0KB)
@2Spfj_e Reference[PDF]
`1 tD&te0 Reference
;aI`4; Service and feedback
=P,h5J Email this page
N:_U2[V^d Add to bookshelf
z 8w&;Ls Add to citation manager
bOY<C%;C
Cite this paper
4mqA*c%6S Email Alert
u lqh}Uv' Relative Information
T({]fc!c Articles with “Koelreuteria bipinnata var. integrifoliola”
o<J_?7c~} Other article of author
&*w)/W · FENG Da-ling
FHoY=fCI · ZHANG Jie
g_T[m* · LIU Xia
e>GX]tK · PENG Wei-xiu
Q\!0V@$ · WU Tong-yan
dx<KZR$!V In vitro culture of immature embryos from Koelreuteria bipinnata
PX5K-|R var. integrifoliola
nMBKZ FENG Da-ling 1, ZHANG Jie 1, LIU Xia 1, PENG Wei-xiu 2* , WU Tong-yan 1
%wc=Mf 1College of Life Science, Agricultural University of Hebei, Baoding 071001, P. R. China
& rsNB:! 2College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding 071001, P. R. China
cb9ndZ)v. f{[ ]m(X; Received Revised Online Accepted
_NkVi_UX Abstract For the mass production of Koelreuteria bipinnata var. integrifoliola with selected, hybrid or genetically engineered genotypes, one potentially desirable propagation strategy is based on embryo culture. The immature embryo development in vitro from K. bipinnata var. integrifoliola was studied under different conditions of embryo age, basic culture media and plant growth regulators. The results show that: 1) germination rate of grade 3 embryos in immature seeds with 0.6–0.8 cm diameter was 98.9%. The germination rate of grade 2 embryos in immature seeds with 0.4–0.6 cm diameter was 77.8% and the germination rate of grade 1 embryos in immature seeds with 0.4 cm diameter was 15.6%. 2) The amounts of macroelements in MS medium had no clear effect on the germination rate of immature grade 3 embryos and had a modest effect on plantlet growth, where the best medium was MS or 1/2 MS. The rates were all greater than 90%. 3) The germination rate of grade 3 embryos was greater than 87% when the medium contained a low concentration of NAA or no plant growth regulators at all and decreased markedly when BAP alone or BAP and NAA together were added to the media. We suggest that in vitro culture of immature embryos from K. bipinnata var. integrifoliola can be enhanced when a small amount of plant growth regulators is added. The addition of BAP has an adverse reaction to the germination and development of immature embryos.
9=-d/y? `.0WK Key words Koelreuteria bipinnata var. integrifoliola immature embryo in vitro culture germination rate
4a]$4LQV DOI:
K~U5jpc Corresponding author
L#\!0YW/@ E-mail:
[email protected] 0/vmj,&B( Authors
Z*M]AvO+# FENG Da-ling 1; ZHANG Jie 1; LIU Xia 1; PENG Wei-xiu 2* ; WU Tong-yan 1
DbL=2 0_A|K>7 =================
McXid~ 谁能帮我下载这个啊!CNKI居然找不到。