l2P=R)@{ :zF, A,) p"ZG%Ow5Q] Forestry Studies in China 2009 11 (3): 179-184 ISSN: 1008-1321 CN: 11-3919/S
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vTzlwK\#1 Articles with “Koelreuteria bipinnata var. integrifoliola”
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}2<7%FL · FENG Da-ling
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M$E: · ZHANG Jie
<3C*Z"aQ>| · LIU Xia
d,n 'n · PENG Wei-xiu
4u 5-7[TZ · WU Tong-yan
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T8DSq In vitro culture of immature embryos from Koelreuteria bipinnata
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&mCJN var. integrifoliola
g~A`N=r;h FENG Da-ling 1, ZHANG Jie 1, LIU Xia 1, PENG Wei-xiu 2* , WU Tong-yan 1
`, Tz Q 1College of Life Science, Agricultural University of Hebei, Baoding 071001, P. R. China
K}MK<2vU 2College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding 071001, P. R. China
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By Nn Received Revised Online Accepted
BeoDKdAwY Abstract For the mass production of Koelreuteria bipinnata var. integrifoliola with selected, hybrid or genetically engineered genotypes, one potentially desirable propagation strategy is based on embryo culture. The immature embryo development in vitro from K. bipinnata var. integrifoliola was studied under different conditions of embryo age, basic culture media and plant growth regulators. The results show that: 1) germination rate of grade 3 embryos in immature seeds with 0.6–0.8 cm diameter was 98.9%. The germination rate of grade 2 embryos in immature seeds with 0.4–0.6 cm diameter was 77.8% and the germination rate of grade 1 embryos in immature seeds with 0.4 cm diameter was 15.6%. 2) The amounts of macroelements in MS medium had no clear effect on the germination rate of immature grade 3 embryos and had a modest effect on plantlet growth, where the best medium was MS or 1/2 MS. The rates were all greater than 90%. 3) The germination rate of grade 3 embryos was greater than 87% when the medium contained a low concentration of NAA or no plant growth regulators at all and decreased markedly when BAP alone or BAP and NAA together were added to the media. We suggest that in vitro culture of immature embryos from K. bipinnata var. integrifoliola can be enhanced when a small amount of plant growth regulators is added. The addition of BAP has an adverse reaction to the germination and development of immature embryos.
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q &AbNWtCV+G Key words Koelreuteria bipinnata var. integrifoliola immature embryo in vitro culture germination rate
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2 DOI:
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vI:C_4| Corresponding author
\ Et 3|Iv E-mail:
[email protected] oEKvl3Hz_ Authors
r:ptQo`1- FENG Da-ling 1; ZHANG Jie 1; LIU Xia 1; PENG Wei-xiu 2* ; WU Tong-yan 1
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